anti-LEFTY2 Antibody [7C5G1H6H10]

7044

Mouse

Human

O00292

O00292

LEFTY2

IgG2a,k

13124811

anticorps

Monoclonal

in solution

[7C5G1H6H10]

Homo sapiens

Protein A Column

unlabelled serum

left-right determination factor 2

ELISA, Western Blot, immuno-histo-chemistry

LEFTY2 antibody can be used in immunohistochemistry starting at 2.5 ug/mL.

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

LEFTY2, EBAF, LEFTYA, Protein lefty-2, LEFTA, TGFB4, Protein lefty-A, TGF-beta-4

A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.

Store LEFTY2 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

LEFTY2 monoclonal antibody was made against recombinant LEFTY2 (Human). Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology.

During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-β family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-β (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-β actions are directed by ligand-induced activation of TGF-β receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-β superfamily, inhibits TGF-β signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-β receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-β-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-β superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 kDa) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.