NCBI GENE nr
Ab raised in
Vial with antibody
ELISA, Western Blot, NELISAut
connective tissue growth factor
CTGF antibody was purified by affinity chromatography employing immobilized CTGF matrix.
CCN2, NOV2, HCS24, IGFBP8, CCN2, Connective tissue growth factor, CCN family member 2, IBP-8
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGF. Human CTGF specific antibody was purified by affinity chromatography employing immobilized hCTGF matrix.
CTGF antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
CTGF (connective tissue growth factor) is a 38kDa, cysteine-rich, secreted peptide. It is a new member of the peptide family that include serum-induced immediate early gene products, a v-src-induced peptide and a putative proto-oncogene. Among the many functions of the CTGF gene family are embryogenesis, wound healing and regulation of extracellular matrix production.
Neutralization:To yield one-half maximal inhibition [ND50] of the biological activity of hCTGF (5.0 ug/mL), a concentration of 6.0 - 9.0 ug/mL of this antibody is required.ELISA:Indirect:To detect hCTGF by indirect ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGF. Sandwich:To detect hCTGF by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required.Western Blot:To detect hCTGF by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGF is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.