NCBI GENE nr

8839

Works with

Human

GENE symbol

WISP2

Antibodie's label

Biotin

Ab raised in

Rabbit

Protein nr

O76076

Ncbi nr

O76076

Omim nr

24211561

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Source

Homo sapiens

Immuno application

ELISA, Western Blot

Antigene name

WNT1 inducible signaling pathway protein 2

Gene names

CCN5, CT58, CTGF-L, CCN5, CTGFL, UNQ228/PRO261, WNT1-inducible-signaling pathway protein 2, CCN family member 5, WISP-2

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Gene info

WISP2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Antigen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGFL/WISP-2. Human CTGFL/WISP-2 specific antibody was purified by affinity chromatography and then biotinylated.

Keep at

WISP-2 antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Antibody advice

ELISA:Direct:To detect hCTGFL/WISP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2.SandwichTo detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human CTGFL/WISP-2 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot:To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.