NCBI GENE nr
Ab raised in
Vial with antibody
ELISA, Western Blot
WNT1 inducible signaling pathway protein 2
CCN5, CT58, CTGF-L, CCN5, CTGFL, UNQ228/PRO261, WNT1-inducible-signaling pathway protein 2, CCN family member 5, WISP-2
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
WISP2 a member of the WNT1 inducible signaling pathway (WISP) protein subfamily, which belongs to the connective tissue growth factor (CTGF) family.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hCTGFL/WISP-2. Human CTGFL/WISP-2 specific antibody was purified by affinity chromatography and then biotinylated.
WISP-2 antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
ELISA:Direct:To detect hCTGFL/WISP-2 by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2.SandwichTo detect hCTGFL/WISP-2 by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 - 1.0 ug/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human CTGFL/WISP-2 as a capture antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hCTGFL/WISP-2. Western Blot:To detect hCTGFL/WISP-2 by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hCTGFL/WISP-2 is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.