NCBI GENE nr

7039

GENE symbol

TGFA

Works with

Human

Omim nr

135689

Ab raised in

Rabbit

Protein nr

P01135

Ncbi nr

P01135

French translation

anticorps

Type

Polyclonal

Vial with antibody

Lyophilized

Source

Homo sapiens

Antibodie's label

unlabelled serum

Immuno application

ELISA, Western Blot, NELISAut

Antigene name

transforming growth factor, alpha

Gene names

TFGAProtransforming growth factor alpha, EGF-like TGF, TGF-alpha

Purity

Anti-hTGF-alpha specific antibody was purified by affinity chromatography employing immobilized hTGF-alpha matrix.

Antigen

Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTGF-alpha (human Transforming Growth Factor-alpha).

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Keep at

TGF-a antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.

Antibody advice

Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTGF-alpha (2 ng/mL), a concentration of 0.1 - 0.15 ug/mL of this antibody is required. ELISA:To detect hTGF-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTGF-alpha. Sandwich:To detect hTGF-a; by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TGF-a; (XP-5281Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-a;. Western Blot:To detect hTGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.