Target of this antibody:

TGF-b2

Antibody host:

Rabbit

Form of antibody:

Liquid

Shipping conditions:

gel pack

Concentration of antibody:

0.5 mg/ml

French translation

anticorps

Isotype:

Rabbit IgG

Clonality of antibody:

Polyclonal

This antibody has been succesfully tested for use in following methods:

Western blot

Purification method:

Affinity purified

This antibody reacts with:

Human, mouse, rat

Storage information:

Shelf time: 12 months in temp.: -20 deg.C

Immunogene sequence:

E. coli expressed recombinant human TGF-β2

How to handle this antibody:

The antibody solution should be gently mixed before use.

Alternative names of antibody target:

TGF-b2, TGF b2 TGF-beta2, TGF beta-2, TGFbeta, TGFb2, transforming growth factor beta 2

Recommended usage:

Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually.

Properties

If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Formulation of this antibody:

100 µg (0.5 mg/ml) affinity purified rabbit polyclonal antibody in phosphate-buffered saline (PBS) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Background information:

The three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2, TGF-beta3) signal throμgh the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and would healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-beta2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development.