Clone:
N/A
Immunogene sequence:
N/A
Target of this antibody:
TGF-b3
Antibody host:
Rabbit
Form of antibody:
Liquid
Shipping conditions:
gel pack
Concentration of antibody:
0.5 mg/ml
French translation
anticorps
Isotype:
rabbit IgG
Clonality of antibody:
Polyclonal
This antibody has been succesfully tested for use in following methods:
Western blot
This antibody reacts with:
Human, Mouse, Rat
Recommended usage:
Western blot: 1-4 µg
Group
Polyclonals and antibodies
Purification method:
Affinity purified rabbit IgG
Storage information:
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
Alternative names of antibody target:
TGF-b3, TGF b3,TGF-beta3, TGF beta-3, TGFbeta, TGFb3, transforming growth factor beta 3
Properties
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Formulation of this antibody:
100 mg (0.5 mg/ml) antigen affinity purified goat polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Background information:
The three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2, TGF-beta3) signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and would healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-beta2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development.