Transforming Growth Factor Beta 1 (TGFb1) Polyclonal Antibody (Mouse), Cy3

anticorps

Mus musculus

Research Use Only.

cy3 conjugation kit

Polyclonals and antibodies

Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.

A Rabbit polyclonal antibody against Mouse Transforming Growth Factor Beta 1 (TGFb1). This antibody is labeled with Cy3.

Sequence of the immunogen: TGFb1 (Ala279~Ser390); Buffer composition: 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol.

Host: Rabbit; Species Reactivity: Mouse; Clonality: polyclonal; Tested applications: WB; Concentration: 500ug/ml; Isotype: IgG; Conjugation: Cy3

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.

If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Transforming Growth Factor Beta 1 (TGFb1) Polyclonal Antibody (Mouse) can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Cloud Clone Corp antibody is well suited for fluorescent microscopy.