Gene name
TGFB4
Gene name synonims
LEFTY2
Immunoglobulin isotype
IgG2a,k
French translation
anticorps
Category
Antibodies
Clonality
Monoclonal
Clone
7C5G1H6H10
Latin name
Mus musculus
Also known as
TGFB4 / LEFTY2
Purification method
Protein A Purified
Host organism
Mouse (Mus musculus)
Subcategory
Mnoclonal antibodies
Concentration
1 miligram per 1 mililiter
Tested applications:
Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
Other gene names
LEFTY2; LEFTY2; EBAF; LEFTA; TGFB4; LEFTYA; EBAF; LEFTA; LEFTYA; TGFB4; TGF-beta-4
Form/Appearance
0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide. Sourced in TCS.
Species reactivity
Human (Homo sapiens); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.
Storage and shipping
For short term storage keep the antibody at +4 degrees Celsius. For longer storage the antibody should be kept at -20 degrees Celsius. Cycles of freezing and defrosting may denaturate the polypeptide chains of the antibodies and reduce their activity.
About
Monoclonals of this antigen are available in different clones. Each murine monoclonal anibody has his own affinity specific for the clone. Mouse monoclonal antibodies are purified protein A or G and can be conjugated to FITC for flow cytometry or FACS and can be of different isotypes.
Specificity and cross-reactivity
A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY. Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Other names
left-right determination factor 2 isoform 1 preproprotein; Left-right determination factor 2; left-right determination factor 2; TGF-beta-4; protein lefty-2; protein lefty-A; left-right determination factor A; transforming growth factor beta-4; endometrial bleeding-associated factor; left-right determination factor 2; Endometrial bleeding-associated factor; Left-right determination factor A; Protein lefty-2; Protein lefty-A; Transforming growth factor beta-4; TGF-beta-4
Properties
If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Human proteins, cDNA and human recombinants are used in human reactive ELISA kits and to produce anti-human mono and polyclonal antibodies. Modern humans (Homo sapiens, primarily ssp. Homo sapiens sapiens). Depending on the epitopes used human ELISA kits can be cross reactive to many other species. Mainly analyzed are human serum, plasma, urine, saliva, human cell culture supernatants and biological samples.