Tested for
FC
Raised in
Mouse
Specificity
Human
Clone
16TFB
Conjugate/Tag
Biotin
Shipping conditions
Blue Ice
French translation
anticorps
Concentration
500 ug/ml
Isotypes
IgG1 kappa
Conjugation
Biotinylated
Category
Primary Antibody
Usage Recommendations
FC: ≤ 0.25 ug/test
Area of research
Cytokines & Growth Factors
Antibody Subtype
Conjugated Monoclonal Antibodies
Storage
Store at 2-8 deg C. Do not freeze. Protect from light.
Form & Buffer
Provided in aqueous buffer with 0.09% NaN3, may contain carrier protein/stabilizer.
Assay Information
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10⁵ to 10⁸ cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Properties
If you buy Antibodies supplied by fitzgerald they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.