Swiss-Prot
P61812
Applications
WB IHC
French translation
anticorps
BiowMW
~ 48; 55 kDa
Reactivity
Human,Mouse,Rat
Group
Polyclonals and antibodies
Application_all
WB: 1:500~1:1000 IHC: 1:50~1:200
Note
For research use only, not for use in diagnostic procedure.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Immunogen
Synthetic peptide, corresponding to amino acids 360-400 of Human TGFβ2.
Specificity
TGF β2 (L388) polyclonal antibody detects endogenous levels of TGF β2 protein.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Usage
Western blot (WB) analysis of TGFβ2 (L388) pAb at 1:500 dilution Lane1:HEK293T whole cell lysate(40ug) Lane2:A375 whole cell lysate(40ug) Lane3:MCF-7 whole cell lysate(40ug)
Alternative Name
Transforming growth factor beta-2; TGF-beta-2; BSC-1 cell growth inhibitor; Cetermin; Glioblastoma-derived T-cell suppressor factor; G-TSF; Polyergin; Latency-associated peptide; LAP; TGFB2
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.