Applications
WB IF
Swiss-Prot
P36897
BiowMW
~ 55 kDa
French translation
anticorps
Reactivity
Human,Mouse,Rat
Group
Polyclonals and antibodies
Application_all
WB: 1:500~1:1000 IF: 1:50~1:200
Note
For research use only, not for use in diagnostic procedure.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Immunogen
Synthetic peptide, corresponding to amino acids 150-200 of Human TGFβ RI.
Specificity
TGFβ RI (E161) polyclonal antibody detects endogenous levels of TGFβ RI protein.
Storage&Stability
Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Purification&Purity
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Usage
Western blot (WB) analysis of TGFβ RI (E161) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:LO2 cell lysate Lane3:Raw264.7 cell lysate Lane4:Mouse kidney tissue lysate Lane5:Rat heat tissue lysate
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
Alternative Name
TGF-beta receptor type-1; TGFR-1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5; ALK-5; ALK5; Serine/threonine-protein kinase receptor R4; SKR4; TGF-beta type I receptor; Transforming growth factor-beta receptor type I; TGF-beta receptor type I; TbetaR-I; TGFBR1; ALK5; SKR4