Reactivity
Human
BiowMW
17 kDa
French translation
anticorps
Swiss-Prot
P01135(Human)
Applications
WB ICC IF IHC IP
Immunogen
Recombinant protein
Purification&Purity
ProA affinity purified
Group
Polyclonals and antibodies
Application_all
WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200
Note
For research use only, not for use in diagnostic procedure.
Specificity
TGFα polyclonal antibody detects endogenous levels of TGFα protein.
Product
Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2
Storage&Stability
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Properties
If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Usage
ICC staining TGF alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Alternative Name
TGF-alpha, ETGF, TGFA, EGF like TGF, EGF-like TGF, ETGF, TFGA, TGF A, TGF type 1, TGF-alpha, Tgfa, TGFA, Transforming growth factor alpha, Transforming growth factor alpha precursor, Wa1, Waved 1,
About
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.