Swiss-Prot

P01137

BiowMW

~ 44 kDa

French translation

anticorps

Applications

WB IHC IF

Reactivity

Human,Mouse,Rat

Group

Polyclonals and antibodies

Application_all

WB: 1:500~1:1000 IHC: 1:50~1:200 IF: 1:50~1:200

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen

Synthetic peptide, corresponding to amino acids 341-390 of Human TGFβ1.

Specificity

TGF β1 (Y369) polyclonal antibody detects endogenous levels of TGF β1 protein.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name

Transforming growth factor beta-1; TGF-beta-1; Latency-associated peptide; LAP; TGFB1; TGFB; TGF β1

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Usage

Western blot (WB) analysis of TGFβ1 (Y369) polyclonal antibody at 1:500 dilution Lane1:L02 whole cell lysate(40ug) Lane2:HEK293T whole cell lysate(40ug) Lane3:AML-12 whole cell lysate(40ug) Lane4:H9C2 whole cell lysate(40ug)

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.