Applications

WB IF

Swiss-Prot

P36897

BiowMW

~ 55 kDa

French translation

anticorps

Reactivity

Human,Mouse,Rat

Group

Polyclonals and antibodies

Application_all

WB: 1:500~1:1000 IF: 1:50~1:200

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Immunogen

Synthetic peptide, corresponding to amino acids 150-200 of Human TGFβ RI.

Specificity

TGFβ RI (E161) polyclonal antibody detects endogenous levels of TGFβ RI protein.

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Usage

Western blot (WB) analysis of TGFβ RI (E161) polyclonal antibody at 1:500 dilution Lane1:Hela cell lysate Lane2:LO2 cell lysate Lane3:Raw264.7 cell lysate Lane4:Mouse kidney tissue lysate Lane5:Rat heat tissue lysate

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Alternative Name

TGF-beta receptor type-1; TGFR-1; Activin A receptor type II-like protein kinase of 53kD; Activin receptor-like kinase 5; ALK-5; ALK5; Serine/threonine-protein kinase receptor R4; SKR4; TGF-beta type I receptor; Transforming growth factor-beta receptor type I; TGF-beta receptor type I; TbetaR-I; TGFBR1; ALK5; SKR4