TGFβ RII (phospho-S225/250) polyclonal antibody

WB

P37173

~ 65 kDa

anticorps

Human,Mouse,Rat

WB: 1:500~1:1000

Polyclonals and antibodies

For research use only, not for use in diagnostic procedure.

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

A synthetic peptide corresponding to residues in Human TGFBR2 around the phosphorylation site of ser225/250.

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

TGF-beta receptor type-2; TGFR-2; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II; TGFBR2

p-TGFBR2 (S225/250) polyclonal antibody detects endogenous levels of human and rat TGFBR2 protein only when phosphorylated at Ser225. This antibody also recognizes mouse TGFBR2 protein only when phosphorylated at Ser250.

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Western blot (WB) analysis of p-TGFBR2 (S225/250) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF(0.1ng/ml,30mins) Lane2:Raw264.7 cell lysate treated with EGF(0.1ng/ml,30mins) Lane3:H9C2 cell lysate treated with EGF(0.1ng/ml,30mins)