Applications

WB

Swiss-Prot

P37173

BiowMW

~ 65 kDa

French translation

anticorps

Reactivity

Human,Mouse,Rat

Application_all

WB: 1:500~1:1000

Group

Polyclonals and antibodies

Note

For research use only, not for use in diagnostic procedure.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Immunogen

A synthetic peptide corresponding to residues in Human TGFBR2 around the phosphorylation site of ser225/250.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Purification&Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Alternative Name

TGF-beta receptor type-2; TGFR-2; TGF-beta type II receptor; Transforming growth factor-beta receptor type II; TGF-beta receptor type II; TbetaR-II; TGFBR2

Specificity

p-TGFBR2 (S225/250) polyclonal antibody detects endogenous levels of human and rat TGFBR2 protein only when phosphorylated at Ser225. This antibody also recognizes mouse TGFBR2 protein only when phosphorylated at Ser250.

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Usage

Western blot (WB) analysis of p-TGFBR2 (S225/250) polyclonal antibody at 1:500 dilution Lane1:HEK293T cell lysate treated with EGF(0.1ng/ml,30mins) Lane2:Raw264.7 cell lysate treated with EGF(0.1ng/ml,30mins) Lane3:H9C2 cell lysate treated with EGF(0.1ng/ml,30mins)