Gene ID number
7039
Conjugation
FITC
Modification site
None
Swiss Prot
P01135
Target Antigen
TGF alpha
Immunogen_range
24-52/160
French translation
anticorps
Modification
Unmodified
Clonality
Polyclonal
Excitation Emission
494nm/518nm
Concentration
1ug per 1ul
Subcellular location
Extracellular
Tested applications
FCM, IF(IHC-P)
Crossreactivity
Human, Mouse, Rat
Additional conjugation
Anti-FITC Antibody
Clone
Polyclonal antibody
Category
Conjugated Primary Antibodies
Host Organism
Rabbit (Oryctolagus cuniculus)
Recommended dilutions
FCM(1:20-100), IF(IHC-P)(1:50-200)
Purification method
This antibody was purified via Protein A.
Long name
TGF alpha Polyclonal Antibody, FITC Conjugated
Synonyms
TFGA; Protransforming growth factor alpha; TGFA
Specificity
This is a highly specific antibody against TGF alpha.
Source
KLH conjugated synthetic peptide derived from human TGF-alpha
Description
The Anti-TGF alpha is a α- or alpha protein sometimes glycoprotein present in blood.
Crossreactive species details
Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.
Storage conditions
Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% sodium azide. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.
Antigen background
TGF alpha is a mitogenic polypeptide that is able to bind to the EGF receptor/EGFR and to act synergistically with TGF beta to promote anchorage-independent cell proliferation in soft agar.
Properties
If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.This Bioss Primary Conjugated Antibodies Fluorescein isothiocyanate (FITC) antibody is currently after some BD antibodies the most commonly used fluorescent dye for FACS. When excited at 488 nanometers, FITC has a green emission that's usually collected at 530 nanometers, the FL1 detector of a FACSCalibur or FACScan. FITC has a high quantum yield (efficiency of energy transfer from absorption to emission fluorescence) and approximately half of the absorbed photons are emitted as fluorescent light. For fluorescent microscopy applications, the 1 FITC is seldom used as it photo bleaches rather quickly though in flow cytometry applications, its photo bleaching effects are not observed due to a very brief interaction at the laser intercept. Bioss Primary Conjugated Antibodies FITC is highly sensitive to pH extremes.