Recognised antigen

CTGF

Uniprot #

P29279

Category

Antibody

Tested applications

WB, IHC-P

French translation

anticorps

Conjugation

Unconjugated

Purity

Antigen affinity

Clone

Polyclonal antibody

Localization

Cytoplasmic, secreted

Form

Antigen affinity purified

Clonality

Polyclonal (rabbit origin)

Host animal

Rabbit (Oryctolagus cuniculus)

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Recommended dilutions

Western blot: 0.1-0.5ug/ml,IHC (Paraffin): 0.5-1ug/ml

Notes

Optimal dilution of the CTGF antibody should be determined by the researcher.

Immunogen

Amino acids 58-349 of human CTGF were used as the immunogen for the CTGF antibody.

Intented use

This CTGF antibodyis to be used only for research purposes and not for diagnostics..

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Species reactivity

Human (Homo sapiens), Rat ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Storage

After reconstitution, the CTGF antibody may be kept for up to one month refrigerated at +4 degrees C.For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Description

CTGF, also known as CCN2 or connective tissue growth factor, is a matricellular protein of the CCN family of extracellular matrix-associated heparin-binding proteins (see also CCN intercellular signaling protein). CTGF has important roles in many biological processes, including cell adhesion, migration, proliferation, angiogenesis, skeletal development, and tissue wound repair, and is critically involved in fibrotic disease and several forms of cancers. The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. And the encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.