Antibody isotype
IgG
Purity
>95%
Immunogen species
Mouse
Species reactivity
Mouse
Tested applications & recommended dilutions
ELISA
Conjugation
Biotin
UniProt ID
P29268
French translation
anticorps
Additional conjugation
Biotinylated
Purification method
Protein G purified
Clonality
Polyclonal antibody
Synonym name
Ctgf Mouse Antibody
Antibody category
Polyclonal Antibodies
Format
Liquid, diluted in buffer
Short name of the antigen
Connective tissue growth factor
Latin name
Mus musculus,Oryctolagus cuniculus
Long name
Rabbit Antibody against Mouse Ctgf, Biotin
Immunogen type
Recombinant Mouse Connective tissue growth factor protein (26-344AA)
Buffer composition
Preservative: 0.03% Proclin 300, Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Alias
Connective tissue growth factor,CCN family member 2,Hypertrophic chondrocyte-specific protein 24,Protein FISP-12,Ctgf,Ccn2,Fisp-12,Fisp12,Hcs24
Description
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.This is against a protein of mus musculus directed antibody.
Shipping and storage
Ctgf Antibody is shipped on ice packs and upon receipt should be stored at -20 or -80 degrees Celsius. We recommend that you prepare the working aliquots in advance so that freez-thaw cycles are avoided. Do not expose the antibodies to drirect bright light, especially the labeled ones as most conjugates are light sensitive.
Background
Major connective tissue mitoattractant secreted by vascular endothelial cells. Promotes proliferation and differentiation of chondrocytes (By similarity). Mediates heparin- and divalent cation-dependent cell adhesion in many cell types including fibroblasts, myofibroblasts, endothelial and epithelial cells (By similarity). Enhances fibroblast growth factor-induced DNA synthesis (By similarity).
About
Rabbits are used for polyclonal antibody production by Cusabio. Rabbit antibodies are very stable and can be stored for several days at room temperature. Cusabio adds sodium azide and glycerol to enhance the stability of the rabbit polyclonal antibodies. Anti-human, anti mouse antibodies to highly immunogenic selected peptide sequences are" monoclonal like" since the epitope to which they are directed is less than 35 amino acids long.
Test
Mouse or mice from the Mus musculus species are used for production of mouse monoclonal antibodies or mabs and as research model for humans in your lab. Mouse are mature after 40 days for females and 55 days for males. The female mice are pregnant only 20 days and can give birth to 10 litters of 6-8 mice a year. Transgenic, knock-out, congenic and inbread strains are known for C57BL/6, A/J, BALB/c, SCID while the CD-1 is outbred as strain.
Properties
If you buy Antibodies supplied by Cusabio they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.