NCBI GENE nr

7044

Ab raised in

Mouse

Works with

Human

Protein nr

O00292

Ncbi nr

O00292

GENE symbol

LEFTY2

Immunoglobulin

IgG2a,k

Omim nr

13124811

French translation

anticorps

Type

Monoclonal

Vial with antibody

in solution

Monoclonal

[7C5G1H6H10]

Source

Homo sapiens

Purity

Protein A Column

Antibodie's label

unlabelled serum

Antigene name

left-right determination factor 2

Immuno application

ELISA, Western Blot, immuno-histo-chemistry

Antibody advice

LEFTY2 antibody can be used in immunohistochemistry starting at 2.5 ug/mL.

Suspention, pH, azide

0.02 M potassium phosphate, 0.15 M sodium chloride, pH 7.2, 0.01% sodium azide.

Gene names

LEFTY2, EBAF, LEFTYA, Protein lefty-2, LEFTA, TGFB4, Protein lefty-A, TGF-beta-4

Spicific to

A BALB/c mouse was immunized with a recombinant form of 6X HIS tagged human LEFTY.

Keep at

Store LEFTY2 antibody at 4 grades C or -20 grades C. As with all antibodies avoid freeze/thaw cycles.

Properties

If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Description

This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.

Antigen

LEFTY2 monoclonal antibody was made against recombinant LEFTY2 (Human). Hybridoma: Produced by the fusion between BALB/c mouse splenocytes and mouse Sp2/0 myeloma cells using conventional hybridoma technology.

Gene info

During vertebrate embryogenesis, a left-right axis is established. Secreted growth factors of the TGF-β family, including gene products derived from nodal, lefty-1 and lefty-2, play crucial roles in establishing left-right asymmetries. TGF-β (Transforming growth factor-beta) is a pleiotropic cytokine that regulates growth and differentiation of diverse types of cells. TGF-β actions are directed by ligand-induced activation of TGF-β receptors. Complexes formed move into the nucleus, where they act as components of a transcriptional complex. Lefty, a novel member of the TGF-β superfamily, inhibits TGF-β signaling. Lefty acts to inhibit phosphorylation of Smad2 following activation of the TGF-β receptor. Lefty also inhibits events downstream from R-Smad phosphorylation. Lefty provides a repressed state of TGF-β-responsive genes. The Lefty family is comprised of Lefty 1 and Lefty 2 in mouse, and Lefty A and Lefty B in humans. Members of the TGF-β superfamily require processing for their activation. Cleavage is therefore an essential step for Lefty activation. Lefty is synthesized as a large inactive precursor (42 kDa) that must be endoproteolytically processed to release the bioactive polypeptide (28 kDa and 34 kDa forms). The 28 kDa form induces MAPK activity.