NCBI GENE nr
Ab raised in
Vial with antibody
ELISA, Western Blot, NELISAut
transforming growth factor, alpha
TFGAProtransforming growth factor alpha, EGF-like TGF, TGF-alpha
Anti-hTGF-alpha specific antibody was purified by affinity chromatography employing immobilized hTGF-alpha matrix.
Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant hTGF-alpha (human Transforming Growth Factor-alpha).
If you buy Antibodies supplied by proscience they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
TGF-a antibody is stable for at least 2 years from date of receipt at -20 grades C. The reconstituted antibody is stable for at least two weeks at 2-8 grades C. Frozen aliquots are stable for at least 6 months when stored at -20 grades C. Avoid repeated freeze-thaw cycles.
Neutralization: To yield one-half maximal inhibition [ND50] of the biological activity of hTGF-alpha (2 ng/mL), a concentration of 0.1 - 0.15 ug/mL of this antibody is required. ELISA:To detect hTGF-alpha by direct ELISA (using 100 μL/well antibody solution) a concentration of at least 0.5 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with compatible secondary reagents, allows the detection of 0.2 - 0.4 ng/well of recombinant hTGF-alpha. Sandwich:To detect hTGF-a; by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.5 - 2.0 ug/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with our Biotinylated Anti-Human TGF-a; (XP-5281Bt) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant hTGF-a;. Western Blot:To detect hTGF-alpha by Western Blot analysis this antibody can be used at a concentration of 0.1 - 0.2 ug/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant hTGF-alpha is 1.5 - 3.0 ng/lane, under either reducing or non-reducing conditions.