Verified reactivity

Human

Raised in

Rabbit

Use before

1 year

Protein number

P36897

Shipping requirements

Blue ice

French translation

anticorps

Clonality

Polyclonal

Estimated production time

3-7 business days

NCBI number

Please refer to NCBI

Gene number

Please refer to GenBank

Purity

Protein G purified,>95%

Group

Polyclonals and antibodies

Notes

For research use only. Not for diagnostic procedures.

Storage recommendation

Aliquot and store at -20°C. Minimize freezing and thawing.

Immunogen

Recombinant human TGF-beta receptor type-1 protein (157-244AA)

Verified applications

ELISA,WB,IHC,IF. Recommended dilution:WB:1:1000-1:5000,IHC:1:20-1:200,IF:1:50-1:200

Properties

If you buy Antibodies supplied by bioma they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Other name

TGF-beta receptor type-1,Activin A receptor type II-like protein kinase of 53kD,Activin receptor-like kinase 5,ALK-5,ALK5,Serine/threonine-p,TGFBR1,ALK5,SKR4

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.

Supplementary information

Transmembrane serine/threonine kinase forming with the TGF-beta type II serine/threonine kinase receptor, TGFBR2, the non-promiscuous receptor for the TGF-beta cytokines TGFB1, TGFB2 and TGFB3. Transduces the TGFB1, TGFB2 and TGFB3 signal from the cell surface to the cytoplasm and is thus regulating a plethora of physiological and pathological processes including cell cycle arrest in epithelial and hematopoietic cells, control of mesenchymal cell proliferation and differentiation, wound healing, extracellular matrix production, immunosuppression and carcinogenesis. The formation of the receptor complex composed of 2 TGFBR1 and 2 TGFBR2 molecules symmetrically bound to the cytokine dimer results in the phosphorylation and the activation of TGFBR1 by the constitutively active TGFBR2. Activated TGFBR1 phosphorylates SMAD2 which dissociates from the receptor and interacts with SMAD4. The SMAD2-SMAD4 complex is subsequently translocated to the nucleus where it modulates the transcription of the TGF-beta-regulated genes. This constitutes the canonical SMAD-dependent TGF-beta signaling cascade. Also involved in non-canonical, SMAD-independent TGF-beta signaling pathways. For instance, TGFBR1 induces TRAF6 autoubiquitination which in turn results in MAP3K7 ubiquitination and activation to trigger apoptosis. Also regulates epithelial to mesenchymal transition through a SMAD-independent signaling pathway through PARD6A phosphorylation and activation.