Target of this antibody:
Form of antibody:
Concentration of antibody:
Clonality of antibody:
This antibody has been succesfully tested for use in following methods:
This antibody reacts with:
Human, Mouse, Rat
Western blot: 1-4 µg
Polyclonals and antibodies
Affinity purified rabbit IgG
Shelf time: 12 months in temp.: -20 deg.C
How to handle this antibody:
The antibody solution should be gently mixed before use.
Alternative names of antibody target:
TGF-b3, TGF b3,TGF-beta3, TGF beta-3, TGFbeta, TGFb3, transforming growth factor beta 3
If you buy Antibodies supplied by Biovision they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.
Formulation of this antibody:
100 mg (0.5 mg/ml) antigen affinity purified goat polyclonal antibody in phosphate-buffered saline (PBS) containing 50% glycerol, 1% BSA, and 0.02% thimerosal.
This antibody needs to be stored at + 4°C in a fridge short term in a concentrated dilution. Freeze thaw will destroy a percentage in every cycle and should be avoided.
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.
The three mammalian isoforms of TGF-beta (TGF-beta1, TGF-beta2, TGF-beta3) signal through the same receptor and elicit similar biological responses. They are multifunctional cytokines that regulate cell proliferation, growth, differentiation and motility as well as synthesis and deposition of the extracellular matrix. They are involved in various physiological processes including embryogenesis, tissue remodeling and would healing. They are secreted predominantly as latent complexes which are stored at the cell surface and in the extracellular matrix. The release of biologically active TGF-beta isoform from a latent complex involves proteolytic processing of the complex and/or induction of conformational changes by proteins such as thrombospondin-1. TGF-beta2 has been shown to exert suppressive effects on IL-2 dependent T-cell growth, and may also have an autocrine function in enhancing tumor growth by suppressing immuno-surveillance of tumor development.