Research Use Only.
cy3 conjugation kit
Polyclonals and antibodies
Sequence of the immunogen: TGFb2 (Ala331~Ser442); Buffer composition: PBS, pH7.4, containing 0.02% NaN3, 50% glycerol.
A Rabbit polyclonal antibody against Rat Transforming Growth Factor Beta 2 (TGFb2). This antibody is labeled with Cy3.
Upon receipt, store at -20°C or -80°C. Prepare working aliqotes prior to storage to avoid repeated freeze-thaw cycles.
Host: Rabbit; Species Reactivity: Rat; Clonality: polyclonal; Tested applications: WB, IHC; Concentration: 500ug/ml; Isotype: IgG; Conjugation: Cy3
Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.
Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.Rats are used to make rat monoclonal anti mouse antibodies. There are less rat- than mouse clones however. Rats genes from rodents of the genus Rattus norvegicus are often studied in vivo as a model of human genes in Sprague-Dawley or Wistar rats.
If you buy Antibodies supplied by Cloud Clone Corp they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Cy3 antibodies are excited by the 488-nanometer wave of an argon laser and the 633-nanometer line of a helium-neon diode laser. 1 of the Transforming Growth Factor Beta 2 (TGFb2) Polyclonal Antibody ( ) can be used in flow cytometry but typically shows lower fluorescence intensity comparable to that of PE or APC. This Cloud Clone Corp antibody is well suited for fluorescent microscopy.