Concentration

N/A

Immunoglobulin isotype

IgG1

Clone

2Ar2

French translation

anticorps

Category

Antibodies

Clonality

Monoclonal

Conjugation

Biotinylated

Subcategory

Mnoclonal antibodies

Host organism

Mouse (Mus musculus)

Source organism

Human (Homo sapiens)

Also known as

Transforming Growth Factor beta1

Other gene names

TGFB1; TGFB1; CED; LAP; DPD1; TGFB; TGFbeta; TGFB

Gene name synonims

TGFB1; TGFB1; CED; LAP; DPD1; TGFB; TGFbeta; TGFB

Gene name

TGFB1; TGFB1; CED; LAP; DPD1; TGFB; TGFbeta; TGFB

Purification method

Affinity Purified by Protein G affinity chromatography.

Form/Appearance

Supplied as a liquid in PBS, pH 7.2. Labeled with Biotin.

Tested applications:

ELISA (EL/EIA), Western Blot (WB), Immunohistochemistry (IHC), Flow Cytometry (FC/FACS), Neutralization

Other names

transforming growth factor beta-1; Transforming growth factor beta-1; transforming growth factor beta-1; TGF-beta-1; TGF-beta 1 protein; latency-associated peptide; transforming growth factor, beta 1; N/A

Species reactivity

Human (Homo sapiens), Mouse (Mus musculus), Rat (Rattus norvegicus); Due to limited knowledge and inability for testing each and every species, the reactivity of the antibody may extend to other species which are not listed hereby.

Storage and shipping

Store the antibody at +4 degrees Celsius for short term storage.. Aliquot to avoid repeated freezing and thawing. For optimal long term storage, the antibody should be kept at -20 degrees Celsius. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Description

Aplha, transcription related growth factors and stimulating factors or repressing nuclear factors are complex subunits of proteins involved in cell differentiation. Complex subunit associated factors are involved in hybridoma growth, Eosinohils, eritroid proliferation and derived from promotor binding stimulating subunits on the DNA binding complex. NFKB 105 subunit for example is a polypetide gene enhancer of genes in B cells.

Properties

If you buy Antibodies supplied by MBS Monoclonals they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.Biotin conjugates can be detected by horseradish peroxidase, alkaline phosphatase substrates or anti biotin conjugated antibodies. Avidin and Streptavidin bind to the small biotin and are couple to HRP or AP for ELISA. To break the streptavidin Biotin bond we suggest to use a 6 molar guanidine HCl solution with acidity of pH 1.6.

Specificity and cross-reactivity

Recognizes human TGF-b1 both in its active form and in its latent form when it is complexed with LAP (latency associated peptide.). Neutralizes the biological activity of TGF-b1 and TGF-b1.2. Species Crossreactivity: mouse and rat. In sandwich ELISA, <2% crossreactivity with recombinant human TGF-b3 and recombinant amphibian TGF-b5 was observed and no crossreactivity with recombinant porcine or recombinant human TGFb2 is observed.; Since it is not possible to test each and every species our knowledge on the corss reactivity of the antibodies is limited. This particular antibody might cross react with speacies outside of the listed ones.