Reactivity

Human

BiowMW

17 kDa

French translation

anticorps

Swiss-Prot

P01135(Human)

Applications

WB ICC IF IHC IP

Immunogen

Recombinant protein

Purification&Purity

ProA affinity purified

Group

Polyclonals and antibodies

Application_all

WB:1:500 ICC:1:50-1:200 IHC:1:50-1:200

Note

For research use only, not for use in diagnostic procedure.

Specificity

TGFα polyclonal antibody detects endogenous levels of TGFα protein.

Product

Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Storage&Stability

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

Properties

If you buy Antibodies supplied by Bioworld they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Usage

ICC staining TGF alpha in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Alternative Name

TGF-alpha, ETGF, TGFA, EGF like TGF, EGF-like TGF, ETGF, TFGA, TGF A, TGF type 1, TGF-alpha, Tgfa, TGFA, Transforming growth factor alpha, Transforming growth factor alpha precursor, Wa1, Waved 1,

About

Polyclonals can be used for Western blot, immunohistochemistry on frozen slices or parrafin fixed tissues. The advantage is that there are more epitopes available in a polyclonal antiserum to detect the proteins than in monoclonal sera.