Conjugation

HRP

Excitation Emission

N/A

Gene ID number

7040

Modification site

None

Swiss Prot

P01137

Tested applications

WB, IHC-P

French translation

anticorps

Modification

Unmodified

Clonality

Polyclonal

Concentration

1ug per 1ul

Immunogen_range

280-330/390

Additional conjugation

HRP Conjugated

Target Antigen

TGF Beta 1+2+3

Crossreactivity

Human, Mouse, Rat

Clone

Polyclonal antibody

Subcellular location

Extracellular, Secreted

Category

Conjugated Primary Antibodies

Host Organism

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

WB(1:100-1000), IHC-P(1:100-500)

Purification method

This antibody was purified via Protein A.

Long name

TGF Beta 1+2+3 Polyclonal Antibody, HRP Conjugated

Source

KLH conjugated synthetic peptide derived from human TGF Beta 1

Synonyms

CED; LAP; DPD1; TGFB; TGFbeta; Transforming growth factor beta-1; TGF-beta-1; TGFB1

Crossreactive species details

Due to limited amount of testing and knowledge, not every possible cross-reactivity is known.

Storage conditions

Keep the antibody in an aqueous buffered solution containing 1% BSA, 50% glycerol and 0.09% Gentamicin. Store refrigerated at 2 to 8 degrees Celcius for up to 1 year.

Specificity

This is a highly specific antibody against TGF Beta 1+2+3. This antibody will preferentially react with TGFB1, but will also detect TGFB2 (93%) and TGFB3 (86%) based upon sequence similarity.

Properties

If you buy Antibodies supplied by Bioss Primary Conjugated Antibodies they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.HRP conjugates are often primary rabbit polyclonal antibodies couples to the enzyme horseradish peroxidase (HRP), found in the roots of horseradish. Also monoclonal HRP conjugates are often used for Western Blot.

Antigen background

Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize TGFB1 and have specific receptors for it. It positively and negatively regulates many other growth factors. It plays an important role in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation in committed osteoblasts. Can promote either T-helper 17 cells (Th17) or regulatory T-cells (Treg) lineage differentiation in a concentration-dependent manner. At high concentrations, leads to FOXP3-mediated suppression of RORC and down-regulation of IL-17 expression, favoring Treg cell development. At low concentrations in concert with IL-6 and IL-21, leads to expression of the IL-17 and IL-23 receptors, favoring differentiation to Th17 cells.