Uniprot #

P36897

Category

Antibody

Tested applications

WB, IHC-P

French translation

anticorps

Conjugation

Unconjugated

Purity

Antigen affinity

Clone

Polyclonal antibody

Form

Antigen affinity purified

Clonality

Polyclonal (rabbit origin)

Recognised antigen

TGFBR1 (TGF beta Receptor I)

Host animal

Rabbit (Oryctolagus cuniculus)

Recommended dilutions

Western blot: 0.5-1ug/ml,IHC (Paraffin): 0.5-1ug/ml

Concentration

0.5mg/ml if reconstituted with 0.2ml sterile DI water

Intented use

This TGFBR1 antibodyis to be used only for research purposes and not for diagnostics..

Properties

If you buy Antibodies supplied by NJS poly they should be stored frozen at - 24°C for long term storage and for short term at + 5°C.

Immunogen

An amino acid sequence from the middle region of human TGF beta Receptor I (DYHEHGSLFDYLNRY) was used as the immunogen for this TGFBR1 antibody (100% homologous in human, mouse and rat).

Notes

The stated application concentrations are suggested starting amounts. Titration of the TGFBR1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Species reactivity

Human (Homo sapiens), Rat, Mouse (Mus musculus) ; Due to limited knowledge and inability to test the antibody against all known species, we cannot guarantee that no other cross reactivity can occur.

Storage

After reconstitution, the TGFBR1 antibody can be stored for up to one month at 4oC. For long-term, aliquot and store at -20 deg. Celcius or lower. Cycles of freezing and thawing can denaturate the peptide chains of the antibodies and reduce their sensitivity and/or change their affinity. Prepare aliqotes in such a manner so that freeze-thaw cycles are minimized. Avoid repeated freezing and thawing.

Additional description

The receptors are ligand binding factors of type 1, 2 or 3 and protein-molecules that receive chemical-signals from outside a cell. When such chemical-signals couple or bind to a receptor, they cause some form of cellular/tissue-response, e.g. a change in the electrical-activity of a cell. In this sense, am olfactory receptor is a protein-molecule that recognizes and responds to endogenous-chemical signals, chemokinesor cytokines e.g. an acetylcholine-receptor recognizes and responds to its endogenous-ligand, acetylcholine. However, sometimes in pharmacology, the term is also used to include other proteins that are drug-targets, such as enzymes, transporters and ion-channels.

Description

Transforming growth factor, beta receptor I is a TGFB receptor. The protein encoded by the TGFBR1 gene forms a heteromeric complex with type II TGF-beta receptors when bound to TGFB, transducing the TGFB signal from the cell surface to the cytoplasm. The encoded protein is a serine/threonine protein kinase. Mutations in this gene have been associated with Loeys-Dietz aortic aneurysm syndrome(LDAS). TGFB1 regulates cell cycle progression by a unique signaling mechanism that involves its binding to TGFBR2 and activation of TGFBR1. Both are transmembrane serine/threonine receptor kinases. TGFBR1 may be inactivated in many of the cases of human tumor cells refractory to TGFB-mediated cell cycle arrest. Vellucci and Reiss(1997) reported that the gene is approximately 31 kb long and contains 9 exons. The organization of the segment of the gene that encodes the C-terminal portion of the serine/threonine kinase domain appears to be highly conserved among members of the gene family.